osteoblast culture medium (obm) enriched with growth supplements  (ScienCell)

Journal: Advanced Healthcare Materials

Article Title: Biological Characterization of Ti6Al4V Additively Manufactured Surfaces: Comparison Between Ultrashort Laser Texturing and Conventional Post‐Processing

doi: 10.1002/adhm.202402873

Figure Lengend Snippet: Representative fluorescent images of osteoblast stained with phalloidin following adhesion onto the different surfaces after 24 h. Images were acquired at 20X magnification with an inverted microscope equipped with an epifluorescence setup (Eclipse TiU, NIKON Europe BV, NITAL SpA, Milano, Italy) (Scale bar: 100 µm).

Article Snippet: The cells were cultivated and expanded in osteoblast basal medium (OBM Osteoblast Growth and Differentiation Basal Medium; LONZA), supplemented with the appropriate additives (OGM Osteoblasts Growth SingleQuots kit, LONZA), 10% fetal bovine serum (FBS, EUROCLONE, Pero, Milano, Italy), 100 U mL −1 penicillin, 100 μg mL −1 streptomycin, (SIGMA, St. Louis, MO) under standard conditions (37 °C, 5%CO 2 /95%air, humidified atmosphere).

Techniques: Staining, Inverted Microscopy

Journal: Advanced Healthcare Materials

Article Title: Biological Characterization of Ti6Al4V Additively Manufactured Surfaces: Comparison Between Ultrashort Laser Texturing and Conventional Post‐Processing

doi: 10.1002/adhm.202402873

Figure Lengend Snippet: Morphometric descriptors of osteoblast shape in response to XZ surfaces sandblasted with corundum (SB‐C), zirconia (SB‐Z), and laser textured (LT).

Article Snippet: The cells were cultivated and expanded in osteoblast basal medium (OBM Osteoblast Growth and Differentiation Basal Medium; LONZA), supplemented with the appropriate additives (OGM Osteoblasts Growth SingleQuots kit, LONZA), 10% fetal bovine serum (FBS, EUROCLONE, Pero, Milano, Italy), 100 U mL −1 penicillin, 100 μg mL −1 streptomycin, (SIGMA, St. Louis, MO) under standard conditions (37 °C, 5%CO 2 /95%air, humidified atmosphere).

Techniques:

Journal: Advanced Healthcare Materials

Article Title: Biological Characterization of Ti6Al4V Additively Manufactured Surfaces: Comparison Between Ultrashort Laser Texturing and Conventional Post‐Processing

doi: 10.1002/adhm.202402873

Figure Lengend Snippet: Metabolic activity was measured with Alamar Blue assay at 3, 7, and 14 days of culture of osteoblasts seeded onto different surfaces finishing conditions realized onto titanium samples with a) XY orientation or b) XZ orientation. The results are given as relative fluorescent units (RFU) and values are reported as mean and [95% CI] obtained from four replicate materials. Statistical comparison is reported in the graphs: among experimental times within the same material three different symbols were used: # for SB‐C; § for SB‐Z and ° for LT (for example # , p < 0.05; ## , p < 0.005; ### , p < 0.001) and among experimental materials within the same experimental time ( * , p < 0.05; ** , p < 0.005; *** , p < 0.001).

Article Snippet: The cells were cultivated and expanded in osteoblast basal medium (OBM Osteoblast Growth and Differentiation Basal Medium; LONZA), supplemented with the appropriate additives (OGM Osteoblasts Growth SingleQuots kit, LONZA), 10% fetal bovine serum (FBS, EUROCLONE, Pero, Milano, Italy), 100 U mL −1 penicillin, 100 μg mL −1 streptomycin, (SIGMA, St. Louis, MO) under standard conditions (37 °C, 5%CO 2 /95%air, humidified atmosphere).

Techniques: Activity Assay, Alamar Blue Assay, Comparison

Journal: Advanced Healthcare Materials

Article Title: Biological Characterization of Ti6Al4V Additively Manufactured Surfaces: Comparison Between Ultrashort Laser Texturing and Conventional Post‐Processing

doi: 10.1002/adhm.202402873

Figure Lengend Snippet: Representative photomicrographs acquired using scanning electron microscopy showing the osteoblast spreading at 7 days onto the two orientations surface finished with two techniques represented by sandblasting with corundum (SB‐C)(a)(d) or zirconia (SB‐Z) (b)(e) and laser texturing (LT)(c)(f).

Article Snippet: The cells were cultivated and expanded in osteoblast basal medium (OBM Osteoblast Growth and Differentiation Basal Medium; LONZA), supplemented with the appropriate additives (OGM Osteoblasts Growth SingleQuots kit, LONZA), 10% fetal bovine serum (FBS, EUROCLONE, Pero, Milano, Italy), 100 U mL −1 penicillin, 100 μg mL −1 streptomycin, (SIGMA, St. Louis, MO) under standard conditions (37 °C, 5%CO 2 /95%air, humidified atmosphere).

Techniques: Electron Microscopy

Journal: Advanced Healthcare Materials

Article Title: Biological Characterization of Ti6Al4V Additively Manufactured Surfaces: Comparison Between Ultrashort Laser Texturing and Conventional Post‐Processing

doi: 10.1002/adhm.202402873

Figure Lengend Snippet: TGF‐β1 and BMP‐2 gene expression at 7 and 14 days of culture of osteoblasts seeded onto different surfaces finishing conditions realized onto titanium samples with XY orientation (a and c) or XZ orientation (b and d). The results show the mean and 95% CI obtained from three replicate materials. Statistical analysis is reported in the graphs: between experimental times in the same material, three different symbols were used: # for SB‐C; § for SB‐Z, and ° for LT (for example ### , p < 0.001; ## , p < 0.005; # , p < 0.05) and among experimental materials in the same experimental time ( *** , p < 0.001; ** , p < 0.005; * , p < 0.05).

Article Snippet: The cells were cultivated and expanded in osteoblast basal medium (OBM Osteoblast Growth and Differentiation Basal Medium; LONZA), supplemented with the appropriate additives (OGM Osteoblasts Growth SingleQuots kit, LONZA), 10% fetal bovine serum (FBS, EUROCLONE, Pero, Milano, Italy), 100 U mL −1 penicillin, 100 μg mL −1 streptomycin, (SIGMA, St. Louis, MO) under standard conditions (37 °C, 5%CO 2 /95%air, humidified atmosphere).

Techniques: Gene Expression

Journal: Advanced Healthcare Materials

Article Title: Biological Characterization of Ti6Al4V Additively Manufactured Surfaces: Comparison Between Ultrashort Laser Texturing and Conventional Post‐Processing

doi: 10.1002/adhm.202402873

Figure Lengend Snippet: ALPL, COL1A1 , and SSP1 gene expression at 7 and 14 days of culture of osteoblasts seeded onto different surfaces finishing conditions realized onto titanium samples with XY orientation (a‐c) or XZ orientation (d‐f). The results show the mean and 95% CI obtained from three replicate materials. Statistical analysis is reported in the graphs: between experimental times in the same material three different symbols were used: # for SB‐C; § for SB‐Z and ° for LT (for example: ### , p < 0.001; ## , p < 0.005; # , p < 0.05) and among experimental materials in the same experimental time ( *** : p < 0.001; ** : p < 0.005; * : p < 0.05).

Article Snippet: The cells were cultivated and expanded in osteoblast basal medium (OBM Osteoblast Growth and Differentiation Basal Medium; LONZA), supplemented with the appropriate additives (OGM Osteoblasts Growth SingleQuots kit, LONZA), 10% fetal bovine serum (FBS, EUROCLONE, Pero, Milano, Italy), 100 U mL −1 penicillin, 100 μg mL −1 streptomycin, (SIGMA, St. Louis, MO) under standard conditions (37 °C, 5%CO 2 /95%air, humidified atmosphere).

Techniques: Gene Expression

Journal: Advanced Healthcare Materials

Article Title: Biological Characterization of Ti6Al4V Additively Manufactured Surfaces: Comparison Between Ultrashort Laser Texturing and Conventional Post‐Processing

doi: 10.1002/adhm.202402873

Figure Lengend Snippet: Quantitative results of ELISA assays performed onto osteoblast's supernatant in response to XZ surfaces sandblasted with corundum (SB‐C), zirconia (SB‐Z), and laser textured (LT).

Article Snippet: The cells were cultivated and expanded in osteoblast basal medium (OBM Osteoblast Growth and Differentiation Basal Medium; LONZA), supplemented with the appropriate additives (OGM Osteoblasts Growth SingleQuots kit, LONZA), 10% fetal bovine serum (FBS, EUROCLONE, Pero, Milano, Italy), 100 U mL −1 penicillin, 100 μg mL −1 streptomycin, (SIGMA, St. Louis, MO) under standard conditions (37 °C, 5%CO 2 /95%air, humidified atmosphere).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Investigating adhesion of primary human gingival fibroblasts and osteoblasts to orthodontic mini-implants by scanning electron microscopy

doi: 10.1038/s41598-024-68486-5

Figure Lengend Snippet: Osteoblast adhesion to miniscrews and controls. Scanning electron micrograph of osteoblast adhesion to different screw areas and controls at 500× magnification. tomas-pin—( a 1 ) Head: polygonal cells with protrusions. ( a 2 ) Neck: polygonal cells with pronounced protrusions and recognizable cell nuclei (arrows). ( a 3 ) Thread: polygonal cells with pronounced protrusions; good networking of the cells is visible (arrow). ( a 4 ) Thread: polygonal and spindle-shaped cells oriented along the grooves. OrthoEasy Pin—( b 1 ) Head: polygonal and spindle-shaped cells. ( b 2 ) Neck: polygonal cells with pronounced protrusions. ( b 3 ) Thread: polygonal and spindle-shaped cells with pronounced protrusions oriented along the grooves. ( b 4 ) Thread: polygonal and spindle-shaped cells aligned along the fine longitudinal grooves and along the overlying transverse grooves (arrow). Dual Top G2—( c 1 ) Head: polygonal cells with protrusions. ( c 2 ) Head: polygonal cells with pronounced protrusions. ( c 3 ) Neck: polygonal cells with protrusions. ( c 4 ) Thread: polygonal and spindle-shaped cells. control samples—( d 1 ) TC coverslips (positive control): spindle-shaped and polygonal cells with numerous extensions; high cell density. ( d 2 ) PTFE (negative control): moderately stretched, spherical cells; only isolated cells.

Article Snippet: Fibroblasts were cultivated in α-MEM (Minimum Essential Medium Eagle, Sigma-Aldrich Chemie GmbH, Hamburg, Germany) and osteoblasts (HOB) in osteoblast basal medium (Osteoblast Basal Medium, PromoCell, Heidelberg, Germany) supplemented with FCS and antibiotics as described in .

Techniques: Control, Positive Control, Negative Control, Isolation